rbt anti p mlc2 t18 s19  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: ZXDC enhances cervical cancer metastasis through IGF2BP3 -mediated activation of RhoA / ROCK signaling

doi: 10.1016/j.isci.2023.107447

Figure Lengend Snippet: ZXDC expression level affects the cytoskeleton reorganization in cervical cancer cells (A) F-actin was stained with phalloidin (Green) in HeLa and SiHa cells with ZXDC overexpression. (B) The filopodia numbers per cell in HeLa and SiHa cells with ZXDC overexpression. (C) The intensity of phalloidin staining was measured used ImageJ in long axis of HeLa and SiHa cells with ZXDC overexpression. (D) F-actin was stained with phalloidin (Green) in HeLa and SiHa cells with ZXDC silencing. (E) The filopodia numbers per cell in HeLa and SiHa cells with ZXDC silencing. (F) The intensity of phalloidin staining was measured used ImageJ in long axis of HeLa and SiHa cells with ZXDC silencing. (G) Phosphorylation levels of cofilin and MLC2 were analyzed by Western blot analysis in indicated cells. Each error bar in (B and E) represents the mean ± SD derived from ten cells. Two-sided Student’s t test was used for all panels. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.

Article Snippet: Rabbit polyclonal anti- p - MLC2 S19 , Cell Signaling Technology , Cat#3671, RRID: AB_330248.

Techniques: Expressing, Staining, Over Expression, Phospho-proteomics, Western Blot, Derivative Assay

Journal: iScience

Article Title: ZXDC enhances cervical cancer metastasis through IGF2BP3 -mediated activation of RhoA / ROCK signaling

doi: 10.1016/j.isci.2023.107447

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti- p - MLC2 S19 , Cell Signaling Technology , Cat#3671, RRID: AB_330248.

Techniques: Virus, Recombinant, Reporter Assay, Chromatin Immunoprecipitation, Sequencing, Gene Expression, shRNA, Software